Journal: Journal of Negative Results in Biomedicine

Article Title: Effects of antibody to receptor activator of nuclear factor κ-B ligand on inflammation and cartilage degradation in collagen antibody-induced arthritis in mice

doi: 10.1186/s12952-014-0018-0

Figure Lengend Snippet: Experimental design. Eight-week-old male DBA1/J mice were divided into 4 groups (6 in each), i.e., RA-/Ab-, RA-/Ab+, RA+/Ab-, and RA+/Ab+. A cocktail of anti-type II collagen antibodies and E. coli LPS was injected intra-peritoneally into the RA+ mice on days 0 and 3, respectively. RA- mice were the control without injection of the anti-type II collagen antibodies and LPS. The OYC1 anti-RANKL monoclonal antibody (5 mg/kg) was injected subcutaneously into the Ab+ mice on day 5. Ab- mice were the control without injection the anti-RANKL antibody. Arthritis scores were determined daily from day 0 to 14. The 4 limbs were removed on day 14 for analysis of histological changes in the joints.

Article Snippet: RA+ mice were injected with a cocktail of antibodies to type II collagen and lipopolysaccharide (LPS) (Chondrex, Inc., Redmond, WA) to induce arthritis.

Techniques: Injection, Control

Journal: Journal of Negative Results in Biomedicine

Article Title: Effects of antibody to receptor activator of nuclear factor κ-B ligand on inflammation and cartilage degradation in collagen antibody-induced arthritis in mice

doi: 10.1186/s12952-014-0018-0

Figure Lengend Snippet: Change in arthritis score after injection of antibodies to type II collagen. Arthritis scores were determined daily after injection of a cocktail of antibodies to type II collagen according to the following criteria: 0, normal; 1, mild but definite redness and swelling of the ankle or wrist, or apparent redness and swelling limited to individual digits regardless of the number of affected digits; 2, moderate redness and swelling of ankle and wrist; 3, severe redness and swelling of the entire paw including digits; 4, maximally inflamed limb with involvement of multiple joints. Data are expressed as the mean ± SD. There was no statistical difference between RA+/Ab- group and RA+/Ab+ group (Steel-Dwass test).

Article Snippet: RA+ mice were injected with a cocktail of antibodies to type II collagen and lipopolysaccharide (LPS) (Chondrex, Inc., Redmond, WA) to induce arthritis.

Techniques: Injection

Journal: Journal of Negative Results in Biomedicine

Article Title: Effects of antibody to receptor activator of nuclear factor κ-B ligand on inflammation and cartilage degradation in collagen antibody-induced arthritis in mice

doi: 10.1186/s12952-014-0018-0

Figure Lengend Snippet: Histological analyses of limb joints. The 4 limbs were excised from all mice at 14 days after injection of a cocktail of antibodies to type II collagen. Decalcified 8-μm sections of hands (A-D) , elbows (E-F) , toes (I-L) , and knees (M-P) from RA-/Ab- (A , E , I , M) , RA-/Ab+ (B , F , J , N) , RA+/Ab- (C , G , K , O) , and RA+/Ab+ (D , H , L , P) mice were stained with hematoxylin and eosin. Synovial thickening and accumulation of inflammatory cells were observed in joints from the RA+ mice (arrowheads in panels C , D , G , H , K , L . O , and P ). Bars: 500 μm (A-D , I-P ) and 200 μm (E-H) .

Article Snippet: RA+ mice were injected with a cocktail of antibodies to type II collagen and lipopolysaccharide (LPS) (Chondrex, Inc., Redmond, WA) to induce arthritis.

Techniques: Injection, Staining